| 免疫初乳中IgG的分离与纯化 |
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| 引用本文： | 范丽娟,潘道东. 免疫初乳中IgG的分离与纯化[J]. 食品科学, 2005, 26(8): 146-149 |
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| 作者姓名： | 范丽娟 潘道东 |
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| 作者单位： | 1. 南京师范大学,生命科学学院,江苏,南京,210097
2. 南京师范大学,金陵女子学院食品科学与营养系,江苏,南京,210097 |
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| 基金项目： | 国家十五奶业重大科技专项（2002BA518A12）;江苏省十五攻关项目（BE2002302）;江苏省自然科学基金（BK2005137） |
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| 摘 要： | 本实验采用硫酸铵一次性盐析、DEAE—Sepharose FF离子交换层析和Sephacryl S-200凝胶过滤层析法，分离纯化免疫初乳中的IgG，并采用SDS—PAGE电泳、单向火箭免疫电泳及试管凝集法对IgG分离物进行了定性定量检测，结果表明所得IgG纯度高，活性大。
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| 关 键 词： | 免疫初乳 IgG 分离纯化 |
| 文章编号： | 1002-6630（2005）08-0146-04 |
| 收稿时间： | 2005-06-18 |
| 修稿时间： | 2005-06-18 |
Isolation and Purification of IgG from Immune Colostrtmas |
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| FAN Li-juan,PAN Dao-dong. Isolation and Purification of IgG from Immune Colostrtmas[J]. Food Science, 2005, 26(8): 146-149 |
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| Authors: | FAN Li-juan PAN Dao-dong |
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| Abstract: | IgG precipitated by ammonium sulfate from Immune colostrum was isolated and purified by DEAE-Sepharose FF Ion exchange chromatography and Sephacryl S-200 Gel filtration chromatography. IgG was validated by SDS-PAGE electrophoresis, one-dimension rocket immunoelectrophoresis and the methods of tube agglutination. The results demonstrated that based on high activity, the purity of IgG was significant increased. |
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| Keywords: | immune colostrums IgG isolation and purification |
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