| 含水量对硅胶柱层析同时分离纯化卵磷脂和脑磷脂的影响 |
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| 引用本文： | 司朝勇,吴素芳,许海丹,吕秀阳. 含水量对硅胶柱层析同时分离纯化卵磷脂和脑磷脂的影响[J]. 高校化学工程学报, 2005, 19(2): 143-147 |
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| 作者姓名： | 司朝勇 吴素芳 许海丹 吕秀阳 |
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| 作者单位： | 浙江大学化学工程与生物工程学系,浙江,杭州,310027;浙江大学化学工程与生物工程学系,浙江,杭州,310027;浙江大学化学工程与生物工程学系,浙江,杭州,310027;浙江大学化学工程与生物工程学系,浙江,杭州,310027 |
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| 基金项目： | 浙江省科技计划(2003C31047)，教育部优秀青年教师资助计划(教人司[2002]40号)项目。 |
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| 摘 要： | 研究了固定相初始含水量和流动相含水量对硅胶柱层析同时分离纯化卵磷脂和脑磷脂的影响。结果表明：含水量对硅胶柱层析同时分离纯化卵磷脂和脑磷脂有显著的影响。硅胶初始含水量在10％、12％(wt)和流动相含水量在0．5％0．9％(vol)范围内，卵磷脂(PC)和脑磷脂(PE)可实现基线分离，PC的回收率可达85％以上，PE的回收率可达70％以上。本研究结果对高纯度卵磷脂和脑磷脂的制备有一定的指导作用。
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| 关 键 词： | 硅胶 柱层析 卵磷脂 脑磷脂 |
| 文章编号： | 1003-9015(2005)02-0143-05 |
| 修稿时间： | 2003-11-18 |
Effects of Water Content on the Simultaneous Purification of PC and PE by Silica-gel Column Chromatography |
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| SI Chao-yong,WU Su-fang,XU Hai-dan,L Xiu-yang. Effects of Water Content on the Simultaneous Purification of PC and PE by Silica-gel Column Chromatography[J]. Journal of Chemical Engineering of Chinese Universities, 2005, 19(2): 143-147 |
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| Authors: | SI Chao-yong WU Su-fang XU Hai-dan L Xiu-yang |
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| Affiliation: | SI Chao-yong,WU Su-fang,XU Hai-dan,L(U) Xiu-yang |
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| Abstract: | The effects of initial water content in silica-gel and the water content of mobile phase on the simultaneous purification of Phosphatidyl Choline(PC) and Phosphatidyl Ethanolamine(PE) by silica-gel column chromatography were studied systematically. The results show that the initial water content in silica-gel and water content of mobile phase have great influence on the purification process. When the initial water content in silica-gel is in the range of 10%~12%(wt) and the water content of mobile phase is 0.5%~0.9%(vol), baseline resolution and higher recovery of PC and PE could be reached. The recovery of PC may be more than 85% and PE over 70%. The results obtained may give some guidances for the preparation of high purity PC and PE. |
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| Keywords: | silica-gel column chromatography phosphatidyl choline phosphatidyl ethanolamine |
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