马来酸酐酰化改性对大豆分离蛋白功能性质的影响

研究马来酸酐酰化改性对大豆分离蛋白功能性质的影响。结果表明:随着马来酸酐用量的增大,大豆分离蛋白的酰化度增大,等电点降低;随着酰化度的增大,大豆分离蛋白构象松散,色氨酸残基的微环境趋向于暴露于水的状态,亲水性增强;经马来酸酐酰化改性后,大豆分离蛋白的溶解性、发泡性、乳化性及乳化稳定性均显著提升,但泡沫稳定性有所下降。研究表明,马来酸酐酰化改性大豆分离蛋白是一种非常有前景的功能性食品添加剂。

Effect of maleylation on functional properties of soy protein isolate

The effect of maleylation on functional properties of soy protein isolate was studied. The results showed that the acylation degree of soy protein isolate increased, and the isoelectric point of soy protein isolate decreased with dosage of maleic anhydride increasing. With acylation degree increasing, the conformation of soy protein isolate was loose, and the microenvironment of tryptophan residue tended to expose to water, and the hydrophilicity of soy protein isolate improved. While maleylation impaired the foam stability, the solubility, foam capacity, emulsifying capacity and emulsion stability of maleylated soy protein isolate were markedly ameliorated. The research showed good potential for maleylated soy protein isolate as functional food additive.