固相萃取柱法分离sn-2位单甘酯的研究

为探寻与气相色谱分析相配套的前处理方法,以高效测定油脂中sn-2位(β位)脂肪酸组成及含量。考察了胰脂酶添加量对甘油三酯酶解程度的影响,并采用Florisil固相萃取柱对酶解产物中sn-2位单甘酯的分离纯化条件进行优化。采用高温气相色谱法分析Florisil固相萃取柱对油脂酶解产物中sn-2位单甘酯的分离能力,结果表明经过Florisil固相萃取法分离得到的sn-2位单甘酯纯度能达到95%;气相色谱法分析常见的7种油脂的sn-2位脂肪酸组成,结果表明Florisil固相萃取法与传统薄层色谱法测得的sn-2位脂肪酸组成不存在显著性差异。

Separation of sn-2 monoacylglycerol by solid phase extraction column method

A pretreatment method matching with gas chromatographic was found to high-efficiently analyze sn-2 fatty acid composition in oil. The effect of dosage of pancreatic lipase on the enzymolysis degree of triglyceride was investigated and the conditions of separation and purification of sn-2 monoacylglycerol in enzymatic hydrolysis product were optimized using a Florisil solid phase extraction column. The ability of Florisil solid phase extraction column separating sn-2 monoacylglycerol was analyzed by high temperature gas chromatography method. The results showed that the purity of sn-2 monoacylglycerol separated by Florisil solid phase extraction column could reach 95%. The sn-2 fatty acids compositions of seven kinds of common oils were analyzed by gas chromatography method, and the results showed that there was no significant differences between the compositions of sn-2 fatty acids obtained by Florisil solid phase extraction method and traditional thin layer chromatography (TLC).