微波法提取菠萝皮中抗氧化活性物质的研究

本实验研究了菠萝皮中总酚、总黄酮的微波提取工艺和不同产地菠萝皮中总酚、总黄酮含量以及提取液抗氧化能力。结果表明:最佳提取工艺条件是微波提取时间160s、微波功率450W、料液比1:40;产于海南、广东、广西三地的菠萝皮中总酚含量分别为979.0、898.1、839.6mg/100g;总黄酮含量分别为882.7、642.4、588.7mg/100g。产于广东、广西、海南的菠萝皮提取液都有较好的抗氧化功能,它们的提取液对DPPH·的清除率分别为87.6%、79.8%、81.6%比对·OH的清除效果更好。

Study on Microwave Extraction and Its Antioxidant Activities of Antioxidant Components from Pineapple Peel

The simultaneous microwave extraction conditions of total phenolics and flavonoids from pineapple peels grown in Hainan,Guangdong and Guangxi areas were optimized via L9(33) orthogonal test,and their antioxidant activities were determined. The results showed that the optimal extraction conditions of both total phenolics and flavonoids are as follows:extraction time 160 s,microwave power 450 W,and ratio of raw material to 75% ethanol as extraction solvent 1:40. The contents of total phenolics in pineapple peels from the three areas are 979.0,898.1 and 839.6 mg/100 g,respectively,and those of total flavonoids are 882.7,642.4 and 588.7 mg/10 g,respectively. All the extracts have better antioxidant activities of scavenging 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical than hydroxyl radical,with the scavenging rates of 87.6%,79.8% and 81.6%.