| 砂箱中柴油降解菌株的分离鉴定 |
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| 引用本文： | 杨金凤,曹冠楠,杨正礼.砂箱中柴油降解菌株的分离鉴定[J].桂林工学院学报,2011(3):450-453. |
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| 作者姓名： | 杨金凤 曹冠楠 杨正礼 |
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| 作者单位： | 中国农业科学院农业环境与可持续发展研究所;中国地质大学(北京)水资源与环境工程北京市重点实验室; |
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| 基金项目： | 科技部国际科技合作项目(2006DFA21180) |
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| 摘 要： | 以0#柴油为唯一碳源,从模拟生物修复柴油污染土壤的砂箱中筛选出4株柴油降解菌株B-1、B-2、B-3和B-4,根据形态学观察及16S rDNA序列比对分析,4株菌株分别属于微杆菌属（Microbacterium sp.）、短波单胞菌属（Brevundimonas sp.）、Tetrathiobacter kashmirensis、假单胞菌属（Pseudomonas sp.）,且菌株B-3是2005年在克什米尔某处果园土壤中新分离出来的柴油降解菌株。此外,通过比较各菌株的生长曲线,菌株B-3和菌株B-4较其他两株菌优先进入对数生长期,对加快柴油的生物降解意义重大。
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| 关 键 词： | 柴油 优势降解菌 16S rDNA测序 聚合酶链反应 |
Isolation and Identification of Diesel Degradation Strain in Sandbox |
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| YANG Jin-feng,CAO Guan-nan,YANG Zheng-li.Isolation and Identification of Diesel Degradation Strain in Sandbox[J].Journal of Guilin University of Technology,2011(3):450-453. |
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| Authors: | YANG Jin-feng CAO Guan-nan YANG Zheng-li |
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| Affiliation: | YANG Jin-feng1,CAO Guan-nan2,YANG Zheng-li1(1.Institute of Agricultural Environment and Sustainable Development,Chinese Academy of Agricultural Sciences,Beijing 100081,China,2.Beijing Key Laboratory of Water Resources & Environmental Engineering,China University of Geosciences,Beijing 100083,China) |
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| Abstract: | Taking 0# diesel oil as the sole carbon source,four diesel-degrading strain(B-1,B-2,B-3 and B-4)are selected from the sand box of diesel contaminated soil.The results of morphological observation and identification of molecular biology show that these strains belong to Microbacterium sp.,Brevundimonas sp.,Pseudomonas sp.,Pseudomonas sp.,respectively.And strain B-3 is a new diesel-degrading strain first found in Kashmir orchard soil in 2005.In addition,strain B-3 and B-4 have preferentially accessed to the l... |
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| Keywords: | diesel dominated degradation strain 16S rDNA sequencing polymerase chain reaction(PCR) |
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