| 产壳聚糖酶菌株的筛选及壳寡糖性质的研究 |
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| 引用本文： | 叶淑红,孙浩,陈丽,闫忠会,曹方,郑婷.产壳聚糖酶菌株的筛选及壳寡糖性质的研究[J].食品研究与开发,2007,28(1):23-26. |
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| 作者姓名： | 叶淑红 孙浩 陈丽 闫忠会 曹方 郑婷 |
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| 作者单位： | 1. 大连轻工业学院生物与食品工程学院,辽宁,大连,116034
2. 长沙环境保护职业技术学院环境检测系,湖南,长沙,410004 |
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| 基金项目： | 辽宁省教育厅资助项目;辽宁省发酵工程重点实验室开放课题 |
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| 摘 要： | 采用透明圈法筛选，从天然土样中获得产壳聚糖酶的野生菌株6株。将其中一株高产壳聚糖酶的微生物菌株命名为BK2。用发酵酶液降解壳聚糖，制备壳寡糖。壳寡糖的抑茵性和溶解性，研究结果表明，壳寡糖对一些常见细菌、霉菌、酵母菌等微生物的生长均有不同程度的抑制作用，它在微酸性的条件下，溶解性及抑菌性较好。
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| 关 键 词： | 壳聚糖酶 筛选 壳寡糖 |
| 修稿时间： | 2006-08-28 |
SCREENING OF STRAINS PRODUCING CHITOSANASE AND STUDY ON PROPERTIES OF CHITOOLIGOSACCHARIDES |
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| YE Shu-hong,SUN Hao,CHEN Li,YAN Zhong-hui,CAO Fang,ZHENG Ting.SCREENING OF STRAINS PRODUCING CHITOSANASE AND STUDY ON PROPERTIES OF CHITOOLIGOSACCHARIDES[J].Food Research and Developent,2007,28(1):23-26. |
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| Authors: | YE Shu-hong SUN Hao CHEN Li YAN Zhong-hui CAO Fang ZHENG Ting |
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| Affiliation: | 1.Biology and Food Engineering School, Dalian Institute of Light Industry, Dalian 116034, Liaoning, China; 2. Department of Environment Monitoring,Changsha Environmental Protection College; Changsha 410004, Hunan, China |
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| Abstract: | Six strains producing chitosanase were screened by brilliant clear circle from soil samples. A strain with high chitosanase activity was named BK2. Chitooligosaccharides was obtained by crude chitosanase solution degradating chitosan. Some properties of chitooligosaccharides were determined. Further reseach results showed theat Chitooligosaccharides inhibited some usual microorganisms to different extent and had certain solubility and bacteriostasis in weak acid. |
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| Keywords: | chitosanase screen chitooligosaccharides |
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