3,3'-diethylthiatricarbocyanine iodide: a highly sensitive chiroptical reporter of DNA helicity and sequence |
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| Authors: | Choi Jung Kyu D'Urso Alessandro Trauernicht Mitch Shabbir-Hussain Murtaza Holmes Andrea E Balaz Milan |
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| Affiliation: | Department of Chemistry, University of Wyoming, Laramie, WY 82071, USA; E-Mails: jchoi4@uwyo.edu (J.K.C.); adurso@unict.it (A.D.); mshabbir@uwyo.edu (M.S.-H.). |
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| Abstract: | Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3'-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)(2) and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal. |
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| Keywords: | Cy7 cyanine dye left-handed Z-DNA circular dichroism DNA recognition sensing |
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