Protective Effect of the Ethyl Acetate Fraction of Sargassum muticum Against Ultraviolet B-Irradiated Damage in Human Keratinocytes |
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Authors: | Piao Mei Jing Yoon Weon Jong Kang Hee Kyoung Yoo Eun Sook Koh Young Sang Kim Dong Sam Lee Nam Ho Hyun Jin Won |
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Affiliation: | School of Medicine, Jeju National University, Jeju 690-756, South Korea; E-Mails: mjpiao@hanmail.net (M.J.P.); pharmkhk@jejunu.ac.kr (H.K.K.); eunsyoo@jejunu.ac.kr (E.S.Y.); yskoh7@jejunu.ac.kr (Y.S.K.). |
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Abstract: | The aim of this study was to investigate the cytoprotective properties of the ethyl acetate fraction of Sargassum muticum (SME) against ultraviolet B (UVB)-induced cell damage in human keratinocytes (HaCaT cells). SME exhibited scavenging activity toward the 1,1-diphenyl-2-picrylhydrazyl radicals and hydrogen peroxide (H(2)O(2)) and UVB-induced intracellular reactive oxygen species (ROS). SME also scavenged the hydroxyl radicals generated by the Fenton reaction (FeSO(4) + H(2)O(2)), which was detected using electron spin resonance spectrometry. In addition, SME decreased the level of lipid peroxidation that was increased by UVB radiation, and restored the level of protein expression and the activities of antioxidant enzymes that were decreased by UVB radiation. Furthermore, SME reduced UVB-induced apoptosis as shown by decreased DNA fragmentation and numbers of apoptotic bodies. These results suggest that SME protects human keratinocytes against UVB-induced oxidative stress by enhancing antioxidant activity in cells, thereby inhibiting apoptosis. |
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Keywords: | Sargassum muticum ultraviolet B reactive oxygen species HaCaT cells apoptosis |
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