Antiproliferative effect of aaptamine on human chronic myeloid leukemia k562 cells |
| |
| Authors: | Jin Meihua Zhao Wennan Zhang Yanwen Kobayashi Motomasa Duan Hongquan Kong Dexin |
| |
| Affiliation: | Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences and Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; E-Mails: jinmeihua@tijmu.edu.cn (M.J.); wennan0719@yahoo.cn (W.Z.); zhangyanwen@tijmu.edu.cn (Y.Z.). |
| |
| Abstract: | We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells. |
| |
| Keywords: | aaptamine K562 cells G2/M arrest p21 |
| 本文献已被 PubMed 等数据库收录！ |
|
