| 应用ELISA检测小鼠肝炎病毒抗体 |
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| 引用本文： | 李建平,屈霞琴,徐蓓,钱琴,沈菊阳,陈天培.应用ELISA检测小鼠肝炎病毒抗体[J].粉末涂料与涂装,1991(3). |
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| 作者姓名： | 李建平 屈霞琴 徐蓓 钱琴 沈菊阳 陈天培 |
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| 作者单位： | 卫生部上海生物制品研究所医学实验动物中心 上海200052 |
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| 摘 要： | 以 DBT 细胞增殖的小鼠肝炎病毒 MHV-3经差速离心浓缩后作为抗原,用辣根过氧化物酶标记的金黄色葡萄球菌 A 蛋白(HRP-SPA)作为第二抗体,通过酶联免疫吸附试验(ELISA)检测 MHV抗体。经本中心及三个外单位共检测362份普通群小鼠血清样本,MHV 抗体检出率为149/362(41.16%)屏障系统内小鼠血清样本121份,均为阴性。
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| 关 键 词： | 小鼠肝炎病毒 酶联免疫吸附试验 |
The Detection of Antibody to Mouse Hepatitis Virus by Enzyme Linked Immunosorbent Assay |
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| Li Jianping Shanghai Instiute of Biological Products.The Detection of Antibody to Mouse Hepatitis Virus by Enzyme Linked Immunosorbent Assay[J].Chinese Journal of Biologicals,1991(3). |
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| Authors: | Li Jianping Shanghai Instiute of Biological Products |
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| Affiliation: | Li Jianping Shanghai Instiute of Biological Products |
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| Abstract: | An ELISA kit for the detection of antibody to mouse hepatits virus (MHV)was established.The antigen was prepared by clarifing the DBT cell cultures infectedwith MHV-3 at different speed by centrifugation.Horseradish oxidase labeled SPA(HRP-SPA) was used as the second antibody.Our center and other three laboratoriesdetected 362 mouse sera from conventional condition and 121 mouse sera in barrier system,with positive rates of 149/362 (41.16%)and zero respectively |
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| Keywords: | MHV ELISA |
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