Participation of sialic acid residue in the fibrinogen-fibrin conversion by thrombin |
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Authors: | S Akihama Y Matsuda T Fukase A Yamanaka M Okude |
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Affiliation: | Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan. |
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Abstract: | In order to estimate the effects of sialic acid residues in fibrinogen on the fibrinogen-fibrin conversion by bovine thrombin the Michaelis constant (Km) and maximum velocity (Vmax) were determined. The Km value obtained by the use of intact-fibrinogen was smaller than that of asialo-fibrinogen. This fact suggests that the sialic acid residues affected the formation of the enzyme-substrate complex. It was also found that in comparison with the asialo-fibrinogen, the intact-fibrinogen was significantly influenced in the gel formation time by the ionic strength in the reaction solution. |
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