Degradable and bioactive scaffold of calcium phosphate and calcium sulphate from self-setting cement for bone regeneration |
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Authors: | Gangfeng Hu Luwei Xiao Hong Fu Dawei Bi Haitao Ma Peijian Tong |
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Affiliation: | (1) The First People’s Hospital of Xiaoshang, 311201 Hangzhou, China;(2) Zhejiang Traditional Chinese Medical University, 310053 Hangzhou, China |
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Abstract: | Calcium sulphate/phosphate cement (CSPC) porous scaffolds were fabricated by introduction of calcium sulphate (CS) into calcium
phosphate cement utilizing particle-leaching method. The morphology, porosity and mechanical strength as well as degradation
of the CSPC scaffolds were characterized. The results reveal that the CSPC with 40 wt% CS content (40 CSPC) scaffolds with
a porosity of 81% showed open macropores with the pore size of 200–500 μm. In addition, the 40 CSPC scaffolds with good degree
of interconnected macropores degraded 60 wt% in Tris–HCl solution after 12 weeks. The proliferation, differentiation and morphology
of MG63 cells on the 40 CSPC scaffolds were determined using MTT assay, ALP activity and SEM. The results suggest that the CSPC scaffolds
could stimulate cell proliferation and differentiation, indicating that CSPC scaffolds were biocompatible and had no negative
effects on the cells in vitro. The CSPC scaffolds were implanted in femur bone defect of rabbits, and the in vivo biocompatibility
and osteogenicity of the scaffolds were investigated. The results indicate that CSPC scaffolds exhibited good biocompatibility,
degradability and osteogenesis in vivo. |
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