| SPA-ELISA用于单核细胞增生性李斯特菌检验的应用研究 |
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| 引用本文： | 姜迪来,凌宗帅,王克刚,张志梅,孙芙蓉,王洪海,项海波,曾萍.SPA-ELISA用于单核细胞增生性李斯特菌检验的应用研究[J].食品科技,2006,31(9):212-215. |
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| 作者姓名： | 姜迪来 凌宗帅 王克刚 张志梅 孙芙蓉 王洪海 项海波 曾萍 |
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| 作者单位： | 1. 淄博出入境检验检疫局,淄博,255031
2. 济南出入境检验检疫局,济南,250014 |
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| 摘 要： | 创造性地将SPA-ELISA应用于食品中单核细胞增生性李斯特菌(简称LM)的检验中,建立了快速检测该菌的ELISA方法。试验表明:该方法可特异性检测肉品中的LM,样品中最低检出量为5cfu/10g,经增菌后的培养液最低检测限可达到105cfu/mL,并可在48h内报告结果,比常规分离培养鉴定方法快5￣6d,适于LM的快速筛选。
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| 关 键 词： | 单核细胞增生性李斯特菌 检测 |
| 文章编号： | 1005-9989(2006)09-0212-04 |
| 修稿时间： | 2006年2月22日 |
Study on application of SPA-ELISA for detection of Listeria Monocytogenes |
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| JIANG Di-lai,LING Zong-shuai,WANG Ke-gang,ZHANG Zhi-mei,SUN Fu-rong,WANG Hong-hai,XIANG Hai-bo,ZENG Ping.Study on application of SPA-ELISA for detection of Listeria Monocytogenes[J].Food Science and Technology,2006,31(9):212-215. |
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| Authors: | JIANG Di-lai LING Zong-shuai WANG Ke-gang ZHANG Zhi-mei SUN Fu-rong WANG Hong-hai XIANG Hai-bo ZENG Ping |
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| Abstract: | An SPA-ELISA was developed for detection of Listeria Monocytogenes(LM) of foodstuff. Testsindicated that the method can specially detect LM in meat, the LOQ was 5 germ per 10 gram sample, which reached 105 cfu/mL after liquid culture. All testing could be finished in 48 hours, which was shorten 5~6 days than routine germ isolating culture and suitable for LM rapidly screening. |
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| Keywords: | SPA-ELISA |
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