气相色谱法测定人参土壤中有机氯农药残留量

建立了人参土壤中9种有机氯农药残留量的气相色谱分析方法.样品用丙酮-石油醚提取,提取液用浓硫酸酸化,经HP-5石英毛细管柱分离后,用GC-ECD内标法定量.9种农药在0.01～1.0 μg/mL范围内线性良好,相关系数均大于0.990 8,样品在2个添加水平时的回收率为82%～108%,相对标准偏差(RSD)为1.3%～8.1%,检出限为0.001～0.002 mg/kg.本文利用该方法对敦化市人参土壤中六六六(HCHs)、滴滴涕(DDTs)和五氯硝基苯(CPCNB)的残留情况进行了分析,结果表明该方法准确可靠、快速灵敏,可用于土壤中残留农药的检测.

Determination of organochlorine pesticides residues in Ginseng soil by chromatography

A gas chromatography(GC)method was developed for the determination of 9 pesticides residues in Ginseng soil. The sample was extracted by mixture of acetone and petroleum ether andacidized by concentrated sulfuric acid. After separated on HP-5 capillary column, the analytes were detected by the electron capture detector. The quantitation for all the compounds was carried out using internal standard method(GC-ECD). The results showed good linearity for each pesticide at the range of 0.01-1.0 μg/mL with correlation coefficient larger than 0.990 8. The recoveries of the pesticides spiked to sample at two levels varied from 82% to 108%, with the repeatability RSD from 1.3% to 8.1%. The detection limits for 9 pesticides were 0.001-0.002 mg/kg. The established method was used to detect the residues of HCHs, DDTs, PCNB in Ginseng soil of Dunhua city. The results show that the method was accurate and reliable, rapid and sensitive, and it can be used for the detection of pesticide residue in the soil.