纳米粒子三明治型传感器的组装及其在BRCA-1检测中的应用 |
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作者单位: | ;1.兰州石化职业技术学院 |
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摘 要: | 采用纳米磁、DNA、纳米金组装三明治型生物传感器。其中,纳米磁粒子(PMPS)用于捕获并分离目标DNA,而被基因探针序列修饰过的纳米金(Au-NPs)则扮演识别序列及产生信号的角色,从而使目标DNA序列量转化为光信号,继而通过紫外分光光度计进行定量分析。这种基于纳米金修饰的DNA探针的新型基因诊断技术,可以用来定量检测分析目标DNA。结果显示,这是一种非常简单经济且实用的检测单个突变基因序列的新方法。
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关 键 词: | 纳米金 纳米磁 三明治型传感器 BRCA-1 基因突变 |
Nanoparticles-fabricated Sandwich Biosensor for Single-mismatched BRCA-1 Detection |
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Affiliation: | ,Department of Applied Chemical Engineering,Lanzhou Petrochemical College of Vocational Technology |
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Abstract: | In this study,a sandwich biosensor consisting of PMP( para- magnetic particles) / DNA /Au- NPs( gold nanoparticles) was fabricated. PMPs captured and separated target DNA while Au- NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Therefore,quantitative information concerning the target analyte was translated into a colorimetric signal,which could be easily and quantitatively measured by low- cost UV- vis spectrophotometric analysis. The results indicated that it was a very simple and economic strategy for detection of single- mismatched DNA strands. |
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Keywords: | gold nanoparticles para-magnetic particles sandwich biosensor BRCA-1 detection single-mismatched |
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