芽孢杆菌ZYCHH-01发酵液提取物的抗菌抗氧化性能评价及应用

由于芽孢杆菌及其细胞外代谢物具有抗氧化、抗菌等性能被用于护肤品中。通过盐酸沉淀法从芽孢杆菌ZYCHH-01发酵产物中提取出活性物质，采用自由基清除率为指标评价提取物的体外抗氧化活性，通过牛津杯扩散实验评估提取物的抗菌能力，通过急性皮肤刺激和人体涂抹实验检测了添加提取物面霜的生物安全性。结果表明，该提取物对DPPH·和ABTS+·具有一定的清除活性，其半清除率（IC50）分别为0.73和0.26 g/L。该提取物对革兰氏阳性菌和阴性菌均有抑制作用，可使细菌形貌受到严重受损。在中国化妆品行业，单一的尼泊金酯最高使用量为0.4wt%，混合酯的最高使用量为总体的0.8wt%，当提取物质量浓度为8 g/L（0.8wt%）时，能有效抑制6种常见的致病菌生长，具有替代市售防腐剂的可行性；且所制备的面霜具有安全性、无皮肤刺激性。

Evaluation of antibacterial and antioxidant properties of Bacillus ZYCHH-01 fermentation extract and its application on facial cream

Bacillus and its extracellular metabolites are used in skin care products due to their antioxidant and antibacterial properties. The active substances were extracted from the fermentation product of Bacillus ZYCHH-01 by hydrochloric acid precipitation method, and the in vitro antioxidant activity of the extract was evaluated by using the free radical scavenging rate as an index, the antibacterial ability of the extract was assessed by Oxford Cup diffusion test, and the biosafety of the extract-added cream was examined by acute skin irritation and human application tests. The results showed that the extract exhibited some scavenging activity against DPPH- and ABTS+- with semi-clearance (IC50) of 0.73 and 0.26 g/L, respectively. The extract inhibited both Gram-positive and negative bacteria and could cause severe damage to the bacterial morphology. In the Chinese cosmetic industry, the maximum amount of single nipagin ester used is 0.4wt%, and the maximum amount of mixed ester used is 0.8wt% of the overall. When the mass concentration of the extract is 8 g/L (0.8wt%), it can effectively inhibit the growth of six common pathogenic bacteria and has the feasibility of replacing commercially available preservatives; and the prepared cream has safety and no skin irritation.