基因组改组技术在微生物育种方面的应用

基因组改组技术是传统微生物菌株改良方法的一种替代方法，该项技术通过基因组水平的递归重组，高效实现整个生物体的定向进化，突破了传统微生物菌株改良方法的局限性。利用基因组改组技术可以在较短时间内得到大量性状优良的正向突变株，具有提高改良菌株产量、增强菌株对环境的耐受性、提高底物利用率等优点，被称为代谢工程和菌株选育发展过程中的一个重要里程碑。该文总结了基因组改组技术的基本概念和一般流程，重点介绍了该项技术在微生物育种方面的应用，并且对该项技术的发展前景进行了展望。

Application of genome shuffling technique in microbial breeding

Genome shuffling technique, an alternative to traditional microbial strain improvement method, can efficiently realize the directed evolution of the entire organism and break through the limitations of traditional microbial strain improvement methods through recursive recombination at the genome level. By this technology, a large number of forward mutants with excellent traits can be obtained in a short period, which has the advantages of increasing improvement strain yield, improving strain tolerance to environment, enhancing substrate utilization. It is called an important milestone in the development of metabolic engineering and strain breeding. In this review, the basic concept and general process of genome shuffling technique were summarized, the application of this technique in microbial breeding was introduced, and the outlook to the future development of this technique was put forward.