腐乳源乳酸乳球菌17M1高密度培养条件研究

为了获得乳酸乳球菌(Lactococcus lactis)17M1的高密度培养方法，以菌体密度和活菌数为评价指标，以豆腐乳清为基础培养基，采用响应面法对该菌株的培养基组成进行优化，并研究其培养条件。结果表明，菌株17M1的最佳培养基组成为：在豆腐乳清中加入2.00%大豆蛋白胨、胰酪蛋白胨1.70%、柠檬酸铵2.30%。在此优化培养基中初始pH值6.15，于37℃培养15 h，乳酸乳球菌17M1的活菌数达到了1.17×10¹⁰CFU/mL，高于M17肉汤培养基活菌数(1.04×10⁹CFU/mL)。该研究结果为乳酸乳球菌17M1的工业化应用提供了技术支持。

High-density culture conditions of Lactococcus lactis 17M1 isolated from sufu

In order to obtain the high-density culture method of Lactococcus lactis 17M1, the medium composition of the strain was optimized by response surface methodology with cell density and viable bacteria number as evaluation indexes and tofu whey as basic medium, and the culture conditions were studied. The results showed that the optimal medium composition of strain 17M1 were as follows: tofu whey was added with 2.00% soybean peptone, casein tryptone 1.70% and triammonium citrate 2.30%. Under the optimal medium with the initial pH 6.15, when cultured at 37 ℃ for 15 h, the viable count of L. lactis 17M1 reached 1.17×1010 CFU/ml, which was higher than the viable bacteria number in the M17 broth medium (1.04×109 CFU/ml). The results provided technical support for the industrial application of L. lactis 17M1.