Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification |
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| Authors: | Shoichiro Yamamoto Tadahide Kurokawa Masashi Sekino Motoshige Yasuike Kenji Saitoh |
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| Affiliation: | 1.National Research Institute of Aquaculture, Fisheries Research Agency, Nikko, Tochigi 321-1661, Japan;2.Tohoku National Fisheries Research Institute, Fisheries Research Agency, Shiogama, Miyagi 985-0001, Japan; E-Mail: ;3.National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Kanagawa 236-8648, Japan; E-Mails: (M.S.); (M.Y.); (K.S.) |
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| Abstract: | We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 and the expected heterozygosity varied from 0.57 to 0.92. Cross-subspecies amplification for O. m. masou, O. m. ishikawae and O. m. subsp. was successful. These microsatellites can be utilized in studies of genetic structure, genetic diversity, and intra- and inter-subspecific hybridization, making a contribution to conservation and management of the Oncorhynchus masou complex. |
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| Keywords: | short tandem repeat simple sequence repeat 454 pyrosequencing next-generation sequencer Oncorhynchus masou |
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