| 亚甲基蓝光度法测定银杏叶提取物的抗氧化活性 |
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| 引用本文： | 郭英.亚甲基蓝光度法测定银杏叶提取物的抗氧化活性[J].云南化工,2007,34(3):38-40. |
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| 作者姓名： | 郭英 |
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| 作者单位： | 郧阳师范高等专科学校,化学系,湖北,丹江口,442700;云南民族大学,化学与生物技术学院,云南,昆明,650031 |
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| 摘 要： | 研究了用亚甲基蓝做显色剂分光光度法测定Co2+-H2O2反应产生羟自由基的方法。产生的羟自由基与亚甲基蓝作用使溶液吸光度降低,利用吸光度的变化间接测定所产生的羟自由基。测定了银杏叶提取物(Gink-go-biloba Extract,EGb)对羟自由基的清除作用。对照实验表明,银杏叶提取物对羟自由基有良好的清除效果,表现出较强的抗氧化活性。
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| 关 键 词： | 亚甲基蓝 羟自由基 抗氧化活性 银杏叶提取物 |
| 文章编号： | 1004-275X（2007）03-0038-03 |
| 修稿时间： | 2006-12-05 |
Spectrophotometric Determination of Antioxidant Activity of the Extract from Ginkgo biloba by Methylene Blue Decolouration |
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| Guo Ying.Spectrophotometric Determination of Antioxidant Activity of the Extract from Ginkgo biloba by Methylene Blue Decolouration[J].Yunnan Chemical Technology,2007,34(3):38-40. |
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| Authors: | Guo Ying |
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| Affiliation: | Department of Chemistry, Yunyang Teachers College, Danjiangkou 442700, China |
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| Abstract: | A method was developed for the determination of the hydroxyl radicals from Co2 -H2O2 reaction by spectrophotometry.Methylene blue was reacted with the produced hydroxyl radicals and the absolution was decreased.The scavenging effects of the extract from Ginkgo biloba leaves on hydroxyl radicals were examined.Result showed that the extract had good scavenging effect on hydroxyl radicals,and the method was rapid,simple and stable. |
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| Keywords: | methylene blue hydroxyl radical antioxidative activity extract of Ginkgo biloba leaves |
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