碳氢化合物残留的生物处理:腐植酸对甲苯降解的影响

研究了腐植酸对荧光假单胞细菌(从土壤分离)降解甲苯的影响。试验采用响应表面方法优化几个环境因素(温度，介质pH值，搅动速率)，试验设计方法为面中心立方体设计，环境因素的优化值由所得试验方程预测。在碳氢化合物降解基质中，加入不同浓度的泥炭腐植酸，测试其对荧光假单胞菌降解甲苯能力的影响。结果表明，腐植酸可以抑制细菌对甲苯的降解。

Biological waste-treatment of hydrocarbon residues: effects of humica acids on the degradation of toluene

The biological degradation potential on toluene of the bacteria Pseudomonas fluorescens isolated from soil, as influenced by humic acids, was investigated. The major environmental factors considered of interest for this experiment were temperature, pH of the media, and agitation (rpm), which were optimized using Response Surface Methodology. A face-centered cube design was adopted for this study, and the optimal values for these factors were pre-dicted by the resulting experimental equations. The degradation of toluene by P. fluorescens was tested by incorporating different concentrations (0.02, 0.04, 0.06, 0.08 and 0.10%) of humic acids, extracted from peat moss, in a hydrocarbon degradation medium. The results showed that the microbial degradation of toluene was inhibited by the presence of humic acids.