| 原生质体融合构建耐高温酵母菌株 |
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| 引用本文： | 孙君社,李雪,李军席. 原生质体融合构建耐高温酵母菌株[J]. 食品与发酵工业, 2002, 28(5): 1-5 |
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| 作者姓名： | 孙君社 李雪 李军席 |
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| 作者单位： | 中国农业大学食品学院,北京,100083 |
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| 基金项目： | 国家自然科学基金资助项目 (No .2 99760 4 5) |
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| 摘 要： | 通过原生质体融合选育耐高温的酵母 ,以解决在纤维素类物质同步发酵过程中酵母发酵温度同纤维素酶酶解温度不一致的问题。利用电场诱导原生质体融合技术对产酒率高的菌株Sb1和产酒率低但耐高温的菌株No 30进行融合。经过融合条件的选择 ,分别对 2亲株进行遗传标记 ,3 5 %蜗牛酶处理原生质体 ,在脉冲强度为 11kV、宽度 10 μS、次数为 2次时进行融合 ,得到数株融合子。鉴定得知 ,融合子 2是所需要的融合子 ,其在固态发酵 45℃时产酒精能力达到体积分数 7 2 8%。
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| 关 键 词： | 酵母菌 原生质体 电融合 高温酒精发酵 |
| 修稿时间： | 2002-01-20 |
Constrution of Thermotolerant Alcohol Producing Yeast by Protoplast Fusion |
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| Sun Junshe Li Xue Li Junxi. Constrution of Thermotolerant Alcohol Producing Yeast by Protoplast Fusion[J]. Food and Fermentation Industries, 2002, 28(5): 1-5 |
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| Authors: | Sun Junshe Li Xue Li Junxi |
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| Abstract: | Protoplast fusion technique between S.cerevisiae No.30 and S.bayanus Sb1 has been studied.No.30 can grow at 45℃ but has a lower alcohol yield. However Sb1 is a high alcohol yield yeast but can't grow at 45℃.The best intensity,width, interval freguency of the electrofusion impulse are 11?kV,10μS,59s respectively. The result shows that the fusant greatly expressed the properties of parent strains in thermotolerance and alcohol yield.The fusant 2 has a high alcohol yield(7.28%)using glucose as substrate at 45℃. |
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| Keywords: | yeast protoplast electrofusion high temperature alcoholic fermentation |
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