| 琥珀酸产生菌原生质体的复合诱变 |
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| 引用本文： | 刘晓艳,林剑,徐世艾. 琥珀酸产生菌原生质体的复合诱变[J]. 食品科技, 2009, 0(3) |
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| 作者姓名： | 刘晓艳 林剑 徐世艾 |
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| 作者单位： | 烟台大学化工制造工程山东省重点实验室; |
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| 基金项目： | 山东省自然基金项目(Y2007B54) |
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| 摘 要： | 利用酶解法制备琥珀酸产生菌的孢子原生质体,对其进行紫外线与He-Ne激光的复合诱变,筛选到了一株突变株MS-23,其琥珀酸产量为1.27g/L,是出发菌株产酸量的20.16倍。将此菌株连续传代5次,具有较好的遗传稳定性。
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| 关 键 词： | 琥珀酸 原生质体 紫外线 He-Ne激光 复合诱变 |
Compound mutagenesis of protoplast from a strain producing succinic acid |
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| LIU Xiao-yan,LIN Jian,XU Shi-ai. Compound mutagenesis of protoplast from a strain producing succinic acid[J]. Food Science and Technology, 2009, 0(3) |
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| Authors: | LIU Xiao-yan LIN Jian XU Shi-ai |
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| Affiliation: | LIU Xiao-yan,LIN Jian,XU Shi-ai (Shandong Key Lab of Chemical Manufacture Engineering,Yantai University,Yantai 264005) |
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| Abstract: | The protoplasts from spores of a strain producing succinic acid were prepared by enzyme and irradiated by both UV and He -Ne laser. After the compound mutation, a high-yielding strain MS-23 was obtained, whose yield of succinic acid is 1.27 g/L, about 20.16 times of its parent. After being propagated for five generations, the mutation strain MS-23 shows a more stable heredity. |
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| Keywords: | succinic acid protoplast UV He-Ne laser compound mutation |
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