| 水产养殖益生菌CW9的鉴定及发酵条件优化 |
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| 引用本文： | 夏振强,朱明.水产养殖益生菌CW9的鉴定及发酵条件优化[J].淮海工学院学报,2014(1):81-85. |
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| 作者姓名： | 夏振强 朱明 |
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| 作者单位： | [1] 中国矿业大学 环测学院,江苏 徐州 222116; 淮海工学院 江苏省海洋生物技术重点实验室,江苏 连云港 222005 [2] 淮海工学院 江苏省海洋生物技术重点实验室,江苏 连云港222005 |
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| 基金项目： | 淮海工学院江苏省海洋生物技术重点实验室开放课题（2011HS006） |
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| 摘 要： | 海水养殖益生菌CW9是一株分离自连云港甲壳类动物养殖水体的益生菌。通过16SrDNA测定和生理生化反应对该菌进行了鉴定,确定为节杆菌属,故该菌命名为Arthrobacter sp.CW9。对培养基配方和培养条件进行优化后的结果为:葡萄糖8g/L,蛋白胨5g/L,酵母膏3g/L,NaCl 10g/L,pH 7.5,温度26℃,接种量为2%以及摇床转速为200r/min,培养21h后,获得最大的细胞浓度达6.90×1010 cfu/mL。与出发条件相比,细胞浓度提升显著,为该菌株的工业化应用奠定了基础。
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| 关 键 词： | 益生菌 鉴定 发酵条件 优化 |
Identification and Optimization of Fermentation Condition of a Mariculture Probiotic Strain CW9 |
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| XIA Zhen-qiang,ZHU Ming.Identification and Optimization of Fermentation Condition of a Mariculture Probiotic Strain CW9[J].Journal of Huaihai Institute of Technology:Natural Sciences Edition,2014(1):81-85. |
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| Authors: | XIA Zhen-qiang ZHU Ming |
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| Affiliation: | 1. School of Environment Science and Spatial Informatics, China University of Mining and Technology, X uzhou 222116, China Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China) |
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| Abstract: | A probiotic strain CW9 isolated from mariculture water was identified by 16S rDNA se-quence alignment and physiological and biochemical characteristic analysis and it was determined to be Arthrobacter genus.The medium components were optimized as follows：glucose 8g/L, peptone 5g/L,yeast abstract 3g/L,NaCl 10g/L,and pH 7.5 with an inoculating volume of 2%.After 21h cultivation at 26 ℃ 200 r/min,the maximum biomass reached 6.90＆#215;1010cfu/mL.The study provides basis for the industrial application of this strain. |
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| Keywords: | probiotics identification fermentation conditions optimization |
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