三相分离技术在食品原料中营养组分提取的应用进展

近年来,在生物分离技术领域人们越来越追求绿色、高效、经济、可扩展的分离方法。三相分离法作为一种新型的非色谱生物分离技术,受到研究人员的广泛关注。随着三相分离技术的不断发展,各种类型的三相分离体系层出不穷,即以叔丁醇为提取剂的传统三相分离体系和以碳酸二甲酯、离子液体、低共熔溶剂为提取剂的其他三相分离体系。此外三相分离法对于成分的提取范围也越来越广泛,不仅涉及油脂、蛋白质、酶、多糖和脂溶性色素等化合物的提取,还可满足多种组分的同时分离,实现对原料样品的高值化利用。因此本文主要综述不同类型的三相分离体系、机制并对目前三相分离法在提取组分中的应用进行归纳,以期为更好地利用三相分离法提取食品组分提供科学的理论参考。

Progress in the application of three-phase partitioning technology in the extraction of nutrient components from food raw materials

In recent years, there has been an increasing pursuit of green, efficient, economical and scalable separation methods in the field of bioseparation technology. As a new type of non-chromatographic biological separation technology, the three-phase partitioning method has received extensive attention from researchers. With the continuous development of three-phase partitioning technology, various types of three-phase partitioning systems have emerged, namely the traditional three-phase partitioning system with tert-butanol as the extractant and other three-phase partitioning systems with dimethyl carbonate, ionic liquids, and low eutectic solvents as the extractant. In addition, the scope of three-phase partitioning methods for the extraction of components is becoming more and more extensive, involving not only the extraction of compounds such as oils, proteins, enzymes, polysaccharides and fat-soluble pigments, but also satisfying the simultaneous separation of multiple components to achieve high value utilization of raw material samples. Therefore, this paper mainly reviews different types of three-phase partitioning systems, mechanisms and summarizes the current applications of three-phase partitioning methods in the extraction of components, in order to provide scientific and theoretical references for optimal utilization of three-phase partitioning methods in the extraction of food components.