甜菜碱微量痕量检测技术研究进展

甜菜碱对动植物营养代谢举足轻重,且具有优良的表面性能和稳定性,广泛应用于化工、饲料、食品、日化、医药等领域,尤其作为一种环境友好的绿色驱油剂在三次采油中具有良好的应用前景。本文对多领域甜菜碱分析技术进行总结,综述了甜菜碱及其衍生物的现行标准检测方法和色谱、质谱及光谱技术研究进展。目前中药材、日化品、食品及农产品中甜菜碱已基本实现低检出限、高灵敏度的定量检测,其中色谱法是国家标准和行业标准常见的仲裁方法,液相色谱-质谱联用技术具有更高的检测精度,但对基质复杂、组分结构未知的甜菜碱样品检测难度仍然较大。紫外分光光度法等光谱技术应用范围较广,但检测精度相对较低。通过对不同方法的性能指标进行比较,结合现有方法应用的局限性对复杂油藏条件下的甜菜碱微量痕量检测趋势进行展望。

Research progress on trace detection technology of betaine

Betaine plays an important role in animal and plant nutrition metabolism, and has excellent surface properties and stability. It is widely used in chemical industry, feed, food, daily chemical, medicine and other fields. Especially, as an environment-friendly chemical flooding agent, betaine has a good application prospect in enhanced oil recovery. The detection techniques of betaine in many fields were summarized and current standard detection methods including chromatographic, mass spectrometry and spectroscopic techniques of betaine and its derivatives were reviewed. At present, the quantitative detection of betaine in Chinese medicinal materials, daily chemical products, food and agricultural products with low detection limit and high sensitivity has been basically realized. Among them, chromatography was a common arbitration method in national and industrial standards. Liquid chromatography-mass spectrometry was considered to be more accurate, but it was still difficult to detect betaine samples with complex substrates and unknown composition. Ultraviolet spectrophotometry and other spectral techniques were widely used, while the detection accuracy was relatively low. By comparing the performance indicators of different methods and considering the limitations of existing methods, the trend of trace betaine detection in complex reservoir conditions was prospected.