乳酸菌对苏尼特羊肠道菌群组成及肉品质的影响

反刍动物肠道菌群多样性对动物肉品质有重要的影响。采用高通量测序技术分析饲粮中添加乳酸菌对苏尼特羊肠道菌群组成及肉品质的影响。选取3月龄健康苏尼特羊12只,分成2组,一组饲喂基础饲粮,另一组在基础饲粮中添加0.5%的乳酸菌,饲养90 d后屠宰。结果表明:在乳酸菌组肠道中拟杆菌门及疣微菌门丰度占比均显著高于对照组(P<0.05),毛螺菌科NK3A20类群及另枝菌属丰度占比均显著低于对照组(P<0.05);在乳酸菌组背最长肌中红度值a^*、亮度值L^*显著高于对照组(P<0.05),风味物质如烃类、酮类、酯类等显著高于对照组(P<0.05),氨基酸以及不饱和脂肪酸含量均显著高于对照组(P<0.05),剪切力值则显著低于对照组(P<0.05)。整体上,饲粮添加乳酸菌可以优化苏尼特羊的肠道菌群结构组成,促进机体营养代谢,进而改善肉品质。

Effects of Lactobacillus on Intestinal Flora Composition and Meat Quality of Sunit Sheep

The diversity of intestinal flora in ruminants plays an important role in the quality of animal meat.In this study,high-throughput sequencing was used to analyze the effects of Lactobacillus on intestinal flora composition and meat quality of Sunit sheep.Twelve 3-month-old healthy Sunit sheep were divided into two groups,one group was fed with basic diet,the other group was fed with 0.5%Lactobacillus and slaughtered after 90 days.The results showed that Bacteroidetes and Verrucomicrobia in the intestinal flora of the Lactobacillus group were significantly higher than the control group(P<0.05),Lachnospiraceae NK3A20 group and Alistipes were significantly lower than the control group(P<0.05).In the Lactobacillus group,the redness value a^*and luminance value L^*were significantly higher than the control group(P<0.05),the flavor substances such as hydrocarbons,ketones and esters were significantly higher than the control group(P<0.05),the contents of amino acids and unsaturated fatty acids were significantly higher than the control group(P<0.05),and the shear force was significantly lower than the control group(P<0.05).Lactobacillus added to feed could optimize intestinal flora composition of Sunit sheep,promote nutrient metabolism of the body and improve meat quality.