超声波辅助提取骆驼皮胶原蛋白的工艺及结构表征

以骆驼皮为原料,研究超声波辅助提取胶原蛋白的最优工艺,并对提取得到的胶原蛋白进行表征.以胶原蛋白的提取率为指标,探讨酶添加量、料液比、超声波功率、超声波处理时间及总提取时间5个因素对骆驼皮胶原蛋白的影响,确定超声波功率后,在单因素实验的基础上,结合响应曲面分析法得到最佳提取条件为:酶添加量4.5％、料液比1:23、超声...

Ultrasonic-assisted Extraction and Structure Characterization of Collagen from Camel Skin

The objective of this study was to obtain the optimal technology of ultrasonic-assisted extraction of collagen from camel skin,and the extracted collagen was characterized and analyzed.In the experiment,the extraction rate of collagen was used as an indicator to explore the effects of enzyme loading,solid-liquid ratio,ultrasonic power,ultrasonic treatment time and total extraction time on camel skin collagen.After determining the ultrasonic power,on the basis of one-factor-at-a-time method,combined with response surface analysis,the best extraction conditions:enzyme loading of 4.5%,solid-liquid ratio of 1∶23,ultrasonic treatment time of 20 min,total extraction time of 51 h,and the actual extraction rate of collagen was(41.99±1.57)%.The SDS-PAGE analysis suggested that the extracted collagen contained three characteristic chains of type I collagen.Ultraviolet spectroscopy analysis was further inferred to be a typical type I collagen.The Fourier transform infrared spectroscopy analysis confirmed that collagen had a complete triple helix structure.Scanning electron microscopy observation revealed that the extracted collagen was in the form of fibrous with intertwined structure.Meanwhile,the Zeta potential of pH 2~9 of collagen was measured,and the potential value showed a downward trend in this range.The isoelectric point was 6.7.