黑菊芋结合酚的提取及其抗氧化活性研究

目的 建立黑菊芋结合态多酚的较优提取工艺,评价其抗氧化活性.方法 采用碱水解结合乙酸乙酯萃取法,以液料比、氢氧化钠水解温度和水解时间为单因素,结合态多酚提取率作为考察指标,采用响应面法优化黑菊芋结合酚的提取工艺;采用福林酚法检测黑菊芋中游离酚和结合酚的含量,并评价其体外抗氧化活性.结果 黑菊芋结合酚的最优提取条件为液料...

Study on extraction and antioxidant activity of bound polyphenols from black Helianthus tuberosus

Objective To establish an optimal extraction process for the bound polyphenols of black Helianthus tuberosus,and evaluate its antioxidant activity.Methods Alkali hydrolysis combined with ethyl acetate extraction method was adopted,the liquid-to-material ratio,sodium hydroxide hydrolysis temperature and hydrolysis time were used as single factors,and the extraction rate of bound polyphenols was used as the investigation index,and the extraction process of black Helianthus tuberosus bound polyphenols was optimized by response surface methodology.Additionally,the folin-ciocalteu method was used to determine the content of free and bound polyphenols and the antioxidant activity in vitro was subsequently evaluated.Results The results suggested that the optimal extraction conditions were as follows:Liquid-to-material ratio 15:1(mL/g),sodium hydroxide hydrolysis time 45 min,hydrolysis temperature 50℃.After extracted with 120 mL ethyl acetate for 10 min,the extraction rate of bound polyphenols was(34.28±1.36)mg/100 g,the predicted value was 33.69 mg/100 g,and the relative error was only 1.75%.The content of free polyphenols in black Helianthus tuberosus was 12.21 times that of bound polyphenols,and its antioxidant activity was correlated with the content of polyphenols.Conclusion The response surface methodology can be used to analyze and predict the extraction process of bound polyphenols from black Helianthus tuberosus.In vitro antioxidant activity of free polyphenols of black Helianthus tuberosus is slightly better than that of bound polyphenols,but the difference is not significant(P>0.05).