| 姜黄素光动力技术对水产食品霍利斯格里蒙特菌和溶藻弧菌的灭活作用 |
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| 引用本文： | 董冬丽,林少玲,孙崇臻,杜文琪,吴希阳,唐书泽. 姜黄素光动力技术对水产食品霍利斯格里蒙特菌和溶藻弧菌的灭活作用[J]. 中国食品学报, 2022, 22(2): 40-48 |
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| 作者姓名： | 董冬丽 林少玲 孙崇臻 杜文琪 吴希阳 唐书泽 |
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| 作者单位： | 暨南大学食品科学与工程系 广州 510632;福建农林大学食品科学学院 福州 350002 |
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| 基金项目： | 国家重点研发计划项目(2018YFC1602504)；闽对外合作项目(2020I0010)；2018广东省重点领域研发计划项目(2019B020212004) |
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| 摘 要： | 目的:研究水溶性姜黄素介导的光动力技术对霍利斯格里蒙特菌和溶藻弧菌的灭活效果及其机理.方法:通过平板菌落计数法测定光动力技术对霍利斯格里蒙特菌和溶藻弧菌的灭活效果,采用试剂盒检测光动力作用后菌体内活性氧水平,利用十二烷基硫酸钠-聚丙稀酰胺凝胶电泳和琼脂糖凝胶电泳分析菌体蛋白降解和基因损伤程度.结果:水溶性姜黄素光动力对...
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| 关 键 词： | 光动力技术 水溶性姜黄素 水产食品 霍利斯格里蒙特菌 溶藻弧菌 |
| 收稿时间： | 2021-07-18 |
Inactivation of Curcumin Photodynamic Technology on Grimontia hollisae and Vibrio alginolyticus in Aquatic Food |
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| Dong Dongli,Lin Shaoling,Sun Chongzhen,Du Wenqi,Wu Xiyang,Tang Shuze. Inactivation of Curcumin Photodynamic Technology on Grimontia hollisae and Vibrio alginolyticus in Aquatic Food[J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22(2): 40-48 |
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| Authors: | Dong Dongli Lin Shaoling Sun Chongzhen Du Wenqi Wu Xiyang Tang Shuze |
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| Affiliation: | (Department of Food Science and Engineering,Jinan University,Guangzhou 510632;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002) |
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| Abstract: | Objectives:To investigate photodynamic technology(PDT)inactivation of Grimontia hollisae(Gh)and Vibrio alginolyticus(Va)mediated by water soluble curcumin and its mechanism.Methods:The inactivation effect of Gh and Va by PDT was determined by colony counting method,reactive oxygen species(ROS)production in bacteria was tested by ROS assay kit,total protein degradation was analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and virulence gene damage was examined by agarose gel electrophoresis.Results:Water soluble curcumin PDT significantly inactivated Gh and Va.The inactivation effects increased with dark incubation time,illumination time and curcumin concentration.After PDT treatment,Gh and Va decreased by 7.98 lg(CFU/mL)and 7.80 lg(CFU/mL),and the inactivation rate was over 99.99%.PDT promoted the ROS level in bacteria to degrade bacteria protein and DNA,but no significant damage to virulence genes of Gh and Va was observed.Conclusion:Curcumin PDT can effectively inactivate Gh and Va via ROS and shows potential application in aquatic food safety. |
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| Keywords: | photodynamic technology(PDT) water soluble curcumin aquatic food Grimontia hollisae(Gh) Vibrio alginolyticus(Va) |
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