食源性致病菌和腐败菌的快速检测方法研究进展

食源性致病菌和腐败菌污染一直是引发食品安全事件的重要因素.传统的检测方法虽具有较高的准确性,但需培养及生化实验,耗费时间长且操作较复杂,开发高效、快速的检测方法对保障食品安全至关重要.近年来,科学技术的进步使一些新方法、新技术逐渐被运用,比传统培养法检测时间更短、效率更高、特异性更好,具有广阔的应用前景.因此,本文综述...

Research progress on methods for the rapid detection of food-borne pathogenic bacteria and spoilage bacteria

Food-borne pathogenic bacteria and spoilage bacteria contamination has always been an important factor in food safety incidents. Traditional microbial detection methods are accurate, however they take long time and need complicated operation, so it is of great significances to develop efficient and fast methods. With scientific and technological development in recent years, many new methods are gradually applied in the detection, they are more effective and specific, the detection time is shorter, and have a wide application. This paper reviews the rapid detection technology of food-borne pathogenic bacteria and spoilage bacteria in recent years, such as paper method, flow cytometry, electrical impedance measurement, adenosine triphosphoric acid bioluminescence techniques, spectroscopic techniques, nucleic acid amplification-based technologies, enzyme-linked immunosorbent assay, biosensors, and so on.The principle, advantages and disadvantages, application progress and the development direction of each method are discussed. It is expected to provide a reference for the prevention and control of food safety incidents caused by food-borne pathogenic bacteria and spoilage bacteria.