酿酒酵母Y3401产己酸乙酯发酵条件的优化

以酿酒酵母Y3401为研究对象,对该菌株发酵产己酸乙酯条件进行优化。首先,通过单因素对其培养基条件(pH值和糖度)及诱导条件(温度、转速、接种量、乙醇添加量、己酸添加量、前体添加时机和诱导时间)进行优化,然后通过Plackett-Burman试验,筛选出5个显著影响酿酒酵母Y3401产己酸乙酯的因素,再通过最陡爬坡试验确定最大响应区域,最后采用Box-Behnken试验设计及响应面分析,确定其最优产己酸乙酯条件为:糖度14 Brix、初始pH 7、温度25℃、转速180 r/min、接种量3.5%、乙醇添加量8%、己酸添加量0.059%、前体添加时机30 h、诱导时间31 h。在此培养条件下己酸乙酯产量达10.1 mg/L。本研究结果有助于酿酒酵母Y3401更好地应用于白酒发酵中。

Optimization of Fermentation Conditions for Ethyl Caproate Production from Saccharomyces cerevisiae Y3401

Saccharomyces cerevisiae Y3401 with high-yield for ethyl caproate was selected,and the fermentation conditions of ethyl caproate from it were optimized.Firstly,the medium conditions(including initial pH value and sugar content)and induction conditions(including temperature,rotation speed,inoculation amount,ethanol addition amoun,caproic acid addition amount,pre-induced period,and post-induced period)were optimized by single factor design.Then,five significant factors for ethyl caproate production were screened by Plackett-Burman design,and the maximum response area was determined by the steepest ascent design.Finally,the optimal fermentation conditions of Y3401 for producing ethyl caproate were obtained by response surface methodology.The optimal conditions were as follows:sugar content 14 Brix,initial pH 7,inoculum size of 3.5%,ethanol content 8%,caproic acid content 0.059%,pre-induced period 30 h,and post-induced period 31 h incubated at 25℃with 180 r/min.Under the condition,the yield of ethyl caproate reached 10.1 mg/L.These results are helpful for the application of S.cerevisiae Y3401 in Baijiu brewing.