高效液相色谱荧光光谱法测定蔬菜中的顺、反白藜芦醇及苷

目的:建立一种灵敏、快速的反相高效液相色谱荧光法,分离和测定蔬菜中的顺、反白藜芦醇及其苷的含量。方法:条件经优化,采用NUCLEODUR100-5C18柱(250mm×4.6mm,5μm);流动相为异丙醇和水二元梯度洗脱系统;流速0.6ml/min;荧光检测器:激发波长:334nm,发射波长:404nm。结果:在选定色谱条件下其顺、反白藜芦醇苷和白藜芦醇的最低检测限分别为0.102、0.117、0.083、0.084ng。线性范围分别为0.004%～1.0mg/L,样品加样平均回收率:反式白藜芦醇及苷分别为94.7%,101.1%;顺式白藜芦醇及苷分别为90.7%,92.4%;相对标准偏差:反式白藜芦醇及苷0.92%～1.32%,顺式白藜芦醇及甘1.25%～1.94%。结论:本方法精密度好,结果可靠,适合蔬菜中顺、反白藜芦苷和白藜芦醇的定量分析。

Quantitative Determination of the (E)-and (Z)-diastereomers of Resveratrol and Resveratrol Glucoside in Vegetables by HPLC and Fluorescence Study

Objecive:A sensitive and rapid high-performance liquid chromatography with fluorescence detector method was developed for the determination of the (E)-and (Z)-diastereomers of resveratrol and resveratrol glucoside in vegetables. Methods: Chromatographic separation was achieved with a NUCLEODUR 100-5 C18 column(250mm×4.6mm, 5μm) while a mobile phase program of gradient elution with is-propyl alcohol and water at a flow rate of 0.6ml/min was employed with fluorescence detector: ex: 260nm and em: 472nm. Results: Under the optimum conditions for every component, the limit of detection of the (Z)-and (E)-diastereomers of resveratrol glucoside and resveratrol was 0.102, 0.117, 0.083 and 0.084 ng respectively at a signal-to-noise 3:1. The linear range was 0.004～1.0mg/L.The average recoveries of the standards added were 94.7% and 101.1%, for the (E)- diastereomers of resveratrol and resveratrol glucoside respectively, and 90.7%, 92.4% for the (Z)-diastereomers of resveratrol and resveratrol glucoside respectively, whereas relative standard deviations were within the range of 0.92%～1.32%, 1.25%～ 1.94% respectivtly. Conclusion: This method is accurate and reliable for the quantitive analysis of the (E)-and (Z)-diastereomers of resveratrol and resveratrol glucoside in vegetables.