酶转化在淫羊藿苷提取中的应用

目前生产淫羊藿苷的过程中,其副产物朝藿定A、B、C等黄酮未被利用好。筛选出了将淫羊藿苷废渣中朝藿定A、B、C等黄酮转化成淫羊藿苷的A.sp.y39菌酶,将其应用于以淫羊藿提取物为原料制备淫羊藿苷的工艺。将150 g西安岳达淫羊藿提取物,溶解于60%乙醇、经D-280脱色柱脱色、结晶得到30.5 g淫羊藿苷;其废渣朝藿定A、B、C混合黄酮,经A.sp.y39菌酶转化,又得到16.6 g淫羊藿苷。共得到纯度90%以上的淫羊藿苷47.1 g;淫羊藿苷的提取率由传统的20.3%提高到31.4%;相比于传统方法,淫羊藿苷的提取率提高了50%以上。本文为节约淫羊藿资源提供了依据。 

Bioconversion utilization of waste-residue from icarrin extraction

In current domestic situation, the by-products containing with flavonoids such as epimedin A, B, C was not being used during the icarrin production from epimedium extract. In this paper, the enzyme from A.sp.y 39 strain was filtered for transformating epimedin A, B, C in waste offscum of epimedium extract. The 150 g of the market epimedium extract was dissolved in 60% ethyl alcohol for decoloring by D-280 macroporous resin, and then 30.5 g of icariin was obtained from above discolored epimedium extract by crystallization method.Then, 16.6 g of icariin was obtained from the waste offscum containing epimedin A, B and C by the enzyme from A.sp.y39 strain.Finally, 47.1 g of icariin ( the purity was over 90%) was obtained from 150 g of epimedium extract by above enzyme transformation methods, the yield of icariin was rised from 20.3% to 31.4%.Compared with the traditional method, the extraction rate of icariin was increased by more than 50%, which provided the basis for saving epimedium resources.