超声与微波辅助提取陈皮中橙皮苷及其抗氧化性研究

将超声波与微波辅助提取法进行对比,旨在确定具有较高提取效率的提取陈皮中陈皮苷的方法,同时评价橙皮苷提取物的抗氧化活性。以1年制陈皮为原材料,橙皮苷为检测指标,考虑料液比、提取时间、提取功率三个因素,通过正交实验优化超声波和微波辅助提取橙皮苷的条件。结果表明,超声波辅助提取橙皮苷的最优工艺条件为:料液比1:50、超声波时间25 min、超声波功率80 W,在该条件下橙皮苷得率是8.06%±0.02%;而微波辅助提取橙皮苷的最优工艺条件为:料液比1:50、微波时间40 s、微波功率为240 W,在该条件下橙皮苷的提取率为8.12%±0.04%。相比于超声辅助提取法,微波辅助提法提取时间短,且提取率高,因此采用微波辅助提取法提取橙皮苷是较有效的方法。通过三种抗氧化体系分析橙皮苷抗氧化活性,结果显示橙皮苷对有机自由基DPPH·、羟自由基·OH有一定的清除作用,对Fe3+也有一定的还原能力。 

Ultrasound and microwave-assisted extracting hesperidin form citrus and study of its antioxidant activities

In this paper, ultrasonic and microwave-assisted extraction methods were compared in order to determine the efficient extraction method, while evaluating the antioxidant activity of hesperidin extract.Single factor and orthogonal design were adopted in the experiments.1-year dried citrus peel was as raw materials and hesperidin was used as detection index, considering solid-liquid ratio, extraction time and extraction power to compare the two methods of extraction process.The results showed that the optimum process conditions of ultrasound-assisted extraction was solid-liquid ratio 1 : 50, ultrasonic time25 min, ultrasonic power 80 W and under this condition the extraction rate was 8.06% ± 0.02%. While optimum process conditions of microwave-assisted extraction was solid-liquid ratio 1 : 50, microwave time 40 s, microwave power 240 W and under this condition the extraction rate was 8.12% ± 0.04%.Compared with the ultrasound-assisted extraction, microwave-assisted extraction takes less time and exhibits more effcient extraction rate, so the method of microwave-assisted extraction was regarded as a more effective way.In addition, it was proved that hesperidin had certain scavenging effect on DPPH· and ·OH, and also had certain reduction effect on Fe3 +.