超声波辅助酶法提取榛蘑多糖

目的:为了研究榛蘑中多糖的提取条件,以榛蘑多糖得率为指标,采用超声波辅助复合酶(纤维素酶、木瓜蛋白酶)法进行实验。方法:通过单因素实验研究了酶解温度、超声功率、超声时间、液料比、酶解时间、复合酶比例以及加酶量对榛蘑多糖得率的影响,在此基础上进行响应面优化实验。结果:通过单因素实验,确定了酶解温度50℃、超声功率360 W、超声时间20 min;通过响应面优化实验,确定了最佳提取条件:加酶量1.9%、复合酶比例2:1、酶解时间138 min、液料比30:1(mL/g)。结论:在此条件下,榛蘑多糖得率为40.56%。 

Ultrasonic-assisted enzymatic method extraction of polysaccharide from Armillaria mellea

Objective: In order to study the extraction technology of polysaccharide from Armillaria mellea, based on the polysaccharide yield, polysaccharide was extracted by ultrasonic-assisted enzymatic ( cellulase, papain) method. Method: This paper studied the optimal extraction conditions by single factor experiment, such as enzymolysis temperature, ultrasonic power, ultrasonic time, liquid material ratio, enzymolysis time, ratio of compound enzyme, enzyme concentration.The optimal conditions were obtained by response surface methodology on the basis of single factor experiment. Result: By single factor experiment, enzymolysis temperature 50 ℃, ultrasonic power 360 W, ultrasonic time 20 min were determined.The results of response surface methodology showed that the optimal extraction conditions were enzyme concentration 1.9%, ratio of compound enzyme 2 : 1, enzymolysis time 138 min, liquid material ratio 30: 1 ( mL/g) .Conclusion: Under the optimal extraction conditions, the yield of polysaccharide reached 40.56%.