冷鲜滩羊肉微生物菌群代谢物与其关联性分析

本文以冷鲜滩羊肉为研究对象,采用选择性培养基得出了宁夏盐池县冷鲜滩羊肉表面初始菌相构成为假单胞菌、肠杆菌、乳酸菌及热死环丝菌。结合课题组成员使用GC-TOF-MS技术所得的微生物差异代谢物,用主成分分析微生物菌群与其关联性。主成分分析的结果表明:与假单胞菌关联程度较大的差异代谢物为D-甘油酸和苯丙氨酸,且代谢物D-甘油酸与苯丙氨酸之间、菌群与代谢物之间均呈现出正相关关系（相关系数矩阵值为正）。而1-磷酸葡萄糖、5’-肌苷酸和乳酸菌关联性较大,且代谢物1-磷酸葡萄糖与5’-肌苷酸之间呈现出正相关关系,而代谢物与菌群呈现出负相关关系（相关系数矩阵值为负）。差异代谢物天冬氨酸、天冬酰胺、乙酰苯胺和核糖与肠杆菌及热死环丝菌菌群相互联系与影响的程度较大,且代谢物天冬酰胺、乙酰苯胺和核糖与菌群之间负相关,而代谢物天冬氨酸与菌群之间正相关;代谢物天冬氨酸与代谢物天冬酰胺、核糖负相关,与乙酰苯胺正相关。本文为揭示菌落变化的内在机制提供理论参考,为冷鲜肉保鲜分子生态学理论构建提供借鉴。 

Correlation analysis of microbial community and their metabolites in cold fresh Tan lamb

In this paper, based on the cold fresh Tan lamb as the research object, using the selective culture method, the initial phases on the chilled meat surface of Tan sheep in Yanchi, Ningxia were determined as Pseudomonas, Enterobacter, Brchothrix thermosphacta and Lactobacillus, and the dominant bacteria was Pseudomonas followed by Enterobacter. The differential metabolites were obtained by gas chromatography time of flight mass spectrometry ( GC-TOF-MS) in combination with the members of the group.Principal component analysis was used to discuss the relationship between the microbial community and their metabolites.The results of principal component analysis showed that it had greater relevance between Pseudomonas and D-glyceric acid and phenylalanine, and the relationships of D-glyceric acid and phenylalanine as well as flora and metabolite had shown a positive correlation tendency. The correlation coefficient matrix was positive. The relevance between glucose-1-phosphate, Inosine-5'-monophosphate and Lactobacillus was significant. Among the metabolites of glucose 1-phosphate and 5'-inosinic acid showed a positive correlation, and the metabolites and bacteria showed a negative correlation. The correlation coefficient matrix was negative. Meanwhile, aspartic acid, asparagine, ribose and acetanilide had great interrelation with Enterobacter and Brchothrix thermosphacta. The metabolites of asparagine, acetanilide had the negative correlation with and ribose and bacterium group, while the aspartic acid and bacterium group had the positive correlation.The metabolite of aspartic acid had negative correlation with asparagine and ribose, positive correlation with acetanilide.This paper provides a theoretical reference for revealing the inner mechanism of colony change, reference for the construction of cold fresh meat molecular ecology theory.