超高压处理对消减乳源酪蛋白抗原性影响的体外模拟研究

目的:研究超高压结合不同模拟胃肠消化对于消减乳源酪蛋白抗原性的影响。方法:以乳源酪蛋白为研究对象,采用不同的压力、温度、保压时间、超高压次数来处理酪蛋白,并对处理后的酪蛋白进行模拟成人、婴儿胃肠消化,用间接酶联免疫吸附法来检测经过处理后酪蛋白过敏原消减效果,并且使用正交设计确定消减酪蛋白过敏原最优处理条件组合。结果:在相同超高压处理条件下,对酪蛋白进行模拟成人、婴儿胃肠消化环境,其结果显示成人比婴儿胃肠消化后酪蛋白过敏原消减的效果更好;高压结合模拟成人胃肠消化的最佳条件组合是:350 MPa、温度30℃、保压时间15 min、高压3次,抑制率为22.67%;高压结合模拟婴儿胃肠消化的最佳条件组合是:350 MPa、温度30℃、保压时间20 min、高压1次,抑制率为39.95%。结论:高压结合不同胃肠消化后都可以消减酪蛋白过敏反应,而且结合成人胃肠消化比结合婴儿胃肠消化对过敏原的消减效果更好。 

Imitative study on the effects of ultra high pressure treatment on the elimination of allergens from bovine casein in vitro

Objective: The effects of ultra high pressure ( UHP) treatment on elimination of allergens in casein from bovine milk were studied by gastrointestinal digestion models.Method: Casein from bovine milk were pretreated at different pressure level, temperature, pressure dwell time and pressure cycle, following by digested at the imitative condition of adult or infant gastrointestinal environment, respectively. The eliminating effectiveness of allergens was detected by indirect enzyme-linked immunosorbent assay ( ELISA) . Orthogonal array design were used to optimize the operating conditions. Result: After the same UHP treatment, the bovine casein was digested in an imitative adult or infant gastrointestinal environment, respetively.The results showed that casein allergen reduction in adult gastrointestinal digestive models was better than in infant's.The optimal UHP combined with adult gastrointestinal digestive models treatment was performed at the condition of 350 MPa, 30 ℃, 15 min, 3 times cycle, and the inhibition rate was 22.67%. The optimal UHP combined with infant gastrointestinal digestive models treatment was performed at the condition of 350 MPa, 30 ℃, 20 min, 1 times cycle, and the inhibition rate was 39.95%.Conclusion: These results demonstrated that UHP treatment followed by adult and infant gastrointestinal digestion had eliminating effect on allergens in casein from bovine milk, and UHP treatment combined with adult gastrointestinal digestion model was more effective in elimination allergens in casein from bovine milk.