基于纳米金标记-适配体识别对鱼中磺胺二甲氧嘧啶的定量检测

纳米金因具有独特的光学效应,已成为极具应用前景的标记材料。适配体对目标分子具有较强特异识别能力,被广泛应用于分析检测领域。本文利用纳米金标记适配体,构建了一种磺胺二甲氧嘧啶的可视化检测新方法。利用该方法对磺胺二甲氧嘧啶进行定量分析,在添加水平分别为2、7 ng/m L下,测得回收率为89.86%和85.64%,日内日间相对标准偏差分别为4.00%、5.28%和5.88%、6.67%。方法的检测线性范围为1~10 ng/m L,检测限为1 ng/m L。以上结果表明该方法准确度高,精密度好,将其应用于鲈鱼、鲶鱼等实际水产样品中磺胺二甲氧嘧啶的定量分析具有可行性。 

Quantitative detection of sulfadimethoxine in fish based on the recognition of gold nanoparticles labeled aptamers

Gold nanoparticles ( Aunps) have been investigated as novel marking materials for their unique luminescent properties.Aptamer is a type of recognition materials with specific binding ability to capture target molecules and have been widely applied in the field of analysis and detection. In our study, a novel detection method for quantitative analysis of sulfadimethoxine was developed based on Au NPs and aptamer.The proposed method was applied to the analysis of real samples.The recoveries were 85.64% and 89.86%, and the relative standard deviation ( RSD) values were 4.00%, 5.28%, and 5.88%, 6.67%, for intra-and inter-day tests, respectively.The linear range of the method was 1¹0 ng/m L, and the detection limit was1 ng/m L.The above results suggested that the proposed method was highly accurate, and precise, and was feasible for the quantification of target sulfadimethoxine in aquatic product.