新型GC固定相的制备及其对手性化合物的拆分研究

合成了纤维素三(3,5-二甲基苯基氨基甲酸酯)并用作气相色谱固定相,制备出手性毛细管柱,考察它的极性、柱效和手性选择性.研究表明,该固定相为中等极性,理论塔板数达1 895 m-1,容量因子1.52.该柱成功地分离了随机选取的25种(包括醇、醚、胺、酯、醛、酮、氨基酸类)外消旋化合物中的20种,其中以(±)-Dihydrocarvylacetate、(±)-Roseoxide、(±)-N-Benzyl-1-phenylethylamine、(±)-Fenchone、DL-Leucine、DL-Isoleucine拆分效果较好,证实该柱具有良好的手性分离性能,可用于气相色谱.

Preparation of Cellulose Tries(3,5-Dimethylphenylcarbamate) Stationary Phase and Separation of Different Kinds of Chiral Compounds in GC

Cellulose tris(3,5-Dimethylphenylcarbamate) stationary phase has been synthesized and prepared for the chiral column of GC.The column efficiency,polarity and selectivity are studied,and the retention mechanism is discuss.Results showed that the polarity of the column is moderate,the column performance is up to 1895 plates/m,and the capacity factor is 1.52.Chiarl compounds,such as alcohols,amine,ketone,ether,ester,amino acides,separate well on the column.It can also be found that six compounds(Roseoxide,Dihydrocarvylacetate,N-Benzyl-1-phenylethylamine,Fenchone,Leucine and Isoleucine) are completely attained with better separation.Among the 25 recemates,20 chiral compounds could be separated on the column.So,the cellulose tris(3,5-Dimethylphenylcarbamate) stationary phase has the recognition for chirality and can be used as chiral stationary phase in GC.