辅酶类物质对长春花细胞合成长春新碱的调控作用

添加5种不同浓度的辅酶类物质，根据单因素实验设计正交实验，研究其对抗肿瘤生物碱长春新碱合成的调控作用，并探讨其作用机理. 结果表明，单独添加每种辅酶类物质对长春新碱的合成均有促进作用，确定其最佳添加浓度为(mmol/L): DL-硫辛酸1.0, VB1 1.5, FMN 3.0, ATP 3.0，赖氨酸3.0，该条件下长春新碱合成量(细胞干重)为0.217 mg/g，为首次在长春花细胞培养中成功合成长春新碱.

Regulating Effect of Coenzyme on Biosynthesis of Vincristine in Catharanthus roseus Cell Suspension Culture

To study the regulating effect of coenzyme on biosynthesis of antitumor alkaloid vincristine, five kinds of coenzyme were added at different concentrations. The regulating mechanism was analyzed. The results showed that adding different coenzymes independently resulted in the increase of vincristine biosynthesis. The optimal adding concentrations were confirmed. Based on the results of coenzyme one-factor experiments, five factors four levels orthogonal experiments were designed. The optimal concentration combination obtained was 1.0 mmol/L DL-thioctic acid, 1.5 mmol/L vitamin B1, 3.0 mmol/L flavin mononucleotide, 3.0 mmol/L adenosine triphosphate, and 3.0 mmol/L lysine. The highest vincristine accumulation reached 0.217 mg/g dry weight. It was the first time for successful biosynthesis of vincristine in Catharanthus roseus cell suspension culture.