| 金黄色葡萄球菌基因组岛对多重耐药表型与生物被膜能力的影响机制 |
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| 引用本文： | 邓阳,梁晏瑞,苗健,李琳,李冰,徐振波.金黄色葡萄球菌基因组岛对多重耐药表型与生物被膜能力的影响机制[J].现代食品科技,2015,31(4):42-50. |
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| 作者姓名： | 邓阳 梁晏瑞 苗健 李琳 李冰 徐振波 |
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| 作者单位： | （1.华南理工大学轻工与食品学院，广东广州 510640）,（1.华南理工大学轻工与食品学院，广东广州 510640）,（1.华南理工大学轻工与食品学院，广东广州 510640）,（1.华南理工大学轻工与食品学院，广东广州 510640）,（1.华南理工大学轻工与食品学院，广东广州 510640）,（1.华南理工大学轻工与食品学院，广东广州 510640）（2.美国马里兰大学微生物病理系，巴尔的摩 MD21201） |
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| 基金项目： | 国家“973”计划项目（2012CB720800）；国家自然科学基金青年基金项目（31201362）；广东省优秀博士学位论文作者资助项目；中央高校基本科研业务费面上项目（2012ZM0060） |
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| 摘 要： | 本文选择常见的典型食源性微生物金黄色葡萄球菌,从耐药性微生物感染防控角度出发,对广州地区临床分离的127株葡萄球菌的耐药表型、基因组岛分型与生物被膜生长能力进行研究。通过微量肉汤稀释法检测确定菌株对26种抗菌药物的药敏结果;PCR扩增葡萄球菌属特异性基因16S r RNA、金黄色葡萄球菌菌株特异性基因fem A、耐药基因mec A以检测确定菌株耐药特性;多重PCR检测金葡菌基因组岛SCCmec基因元件中ccr复合物,mec复合物以对其进行分型。107株耐药型金葡菌均为多重耐药,且呈耐9种或以上抗生素占76.1%(86/113)。113株葡萄球菌SCCmec分型结果为:I型0株,II型12株,III型73株,IV型10株,V型11株,5株为无法分型;本文对基因组岛和金黄色葡萄球菌耐药表型与生物被膜能力的相关性进行分析与探讨,为进一步对各种食源性微生物引起的食品污染进行安全控制,提供了研究基础。
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| 关 键 词： | 金黄色葡萄球菌 多重耐药性 SCCmec基因元件 生物被膜 防控措施 |
| 收稿时间： | 2014/7/24 0:00:00 |
Impact Mechanism of Staphylococcus aureus Genomic Islands on Multi-drug Resistant Phenotype and Biofilm-forming Capacity |
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| DENG Yang,LIANG Yan-rui,MIAO Jian,LI Lin,LI Bing and XU Zhen-bo.Impact Mechanism of Staphylococcus aureus Genomic Islands on Multi-drug Resistant Phenotype and Biofilm-forming Capacity[J].Modern Food Science & Technology,2015,31(4):42-50. |
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| Authors: | DENG Yang LIANG Yan-rui MIAO Jian LI Lin LI Bing and XU Zhen-bo |
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| Affiliation: | (1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China),(1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China),(1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China),(1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China),(1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) and (1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China)
(2.Department of biomedical science of university of Maryland, Baltimore 21201, United States) |
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| Abstract: | Staphylococcus aureus (S.aureus) has been long recognized as a common food-borne pathogen in food safety. To study their drug resistence, genomic island typing and biofilm-foming capacity, a toal of 127 clinical isolates of staphylococci strains from Guangzhou were subject to the investigation. A detection of Staphylococcus aureus was conducted with a Micro-broth dilution method to determine the strain susceptibility results from 26 kinds of antibiotics. S.aureus was further confirmed with the detection of specific 16S rRNA, femA, mecA genes by PCR. Ccr and mec complexes of S.aureus SCCmec genomic island elements were typed by multi-PCR. In this experiment, 107 drug-resistent staphylococci are all multi-drug resistant strains, and 76.1% (86/113) show resistance to nine kinds of antibiotics or more. 113 Staphylococcus SCCmec typing results: typeI (0 strains), typeII (12 strains), typeIII (73 strains), typeIV (10 strains), typeV (11 strains),5strains is not typing. In this paper, the correlation of genomic islands with Staphylococcus aureus drug-resistance phenotype and biofilm-forming capacity is analyzed and discussed. It provides a research base for further security control on a variety of food contamination caused by food-borne microorganisms. |
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| Keywords: | Staphylococcus aureus muti-drug resistance SCCmec genomic elements biofilm prevention measures |
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