Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides |
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| Authors: | T. N. Seyfried E. J. Weber R. K. Yu |
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| Affiliation: | (1) Department of Neurology, Yale University School of Medicine, 06510 New Haven, CT;(2) Agricultural Research Service U.S. Department of Agriculture, University of Illinois, 230 Davenport Hall, 61801 Urbana, IL;(3) Department of Neurology, Yale University School of Medicine, 06510, CT, New Haven |
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| Abstract: | Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides. |
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