Cholesterol content of human parotid saliva |
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| Authors: | Gert M. Jacobsohn Raf Ael J. Montiel |
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| Affiliation: | (1) Department of Biological Chemistry, Hahnemann Medical College, 19102 Philadelphia, Pennsylvania;(2) Present address: Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela |
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| Abstract: | Free and esterifed cholesterol were determined in parotid fluid collected from 19 individuals by encapsulation of Stenson's duct in the oral 16.7±7.1 (SD) μg/100 ml and cholesterol esters averaged 13.2±8.0 (SD) μg/100 ml. Two assays are described for the measurement of cholesterol in parotid saliva. Lipophilic material was extracted with ether-ethanol (3∶1). In assay 1, the solid residue of the extract was separated into its components by thin layer chromatography. Substances on the chromatogram were made to fluoresce and the spots were photographed. Cholesterol was determined by densitometry of its black image on film. In assay 2, the ether-ethanol residue was subjected to quantitative gas chromatography. Both methods yield similar data. |
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