首页 | 本学科首页   官方微博 | 高级检索  
     


Rapid detection of bacterial cell from whole blood: Integration of DNA sample preparation into single micro-PCR chip
Authors:Kyu-Youn HwangAuthor Vitae  Sung-Young JeongAuthor VitaeYoung-Rok KimAuthor Vitae  Kak NamkoongAuthor VitaeHee-Kyun LimAuthor Vitae  Won-Seok ChungAuthor VitaeJoon-Ho KimAuthor Vitae  Nam HuhAuthor Vitae
Affiliation:a Bio & Health Lab., Samsung Advanced Institute of Technology (SAIT), Samsung Electronics Co., Yongin-Si, Gyeonggi-Do 446-712, Republic of Korea
b Interdisciplinary Program of Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea
c Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, Kyunghee University, Yongin-Si, Gyeonggi-Do 446-701, Republic of Korea
d Department of Bio and Brain Engineering, College of Life Science and Bioengineering, KAIST, 335 Gwanhangno, Yuseong-gu, Daejeon 305-701, Republic of Korea
Abstract:A novel bacterial cell detection method from blood samples has been developed for molecular diagnostics. Functional integration of DNA sample preparation into polymerase chain reaction (PCR) chip enabled detection of pathogenic bacterial cells in a single microchip. Surface-modified micropillars possessing affinity for bacterial cells were fabricated inside a PCR chip, and reaction conditions were optimized to render the microchip with high surface-to-volume ratio PCR-compatible. After bacterial cells were captured on the micropillars from whole blood and PCR inhibitors were washed out, PCR mixture was injected to allow real-time amplification of DNA extracted from the isolated cells. Cell enrichment effect produced by volume reduction from large initial sample to small micro-PCR chip chamber led to increased detection sensitivity. Moreover, the developed method from sample preparation to detection of bacterial cells from whole blood took less than 1 h. These results demonstrated that the surface-modified pillar-packed microchip would be a practical approach for integration into Lab-On-a-Chip (LOC) to enable point-of-care genetic analysis.
Keywords:Micro-PCR  DNA sample preparation  Integration  Bacteria
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号