A simple method for the acquisition of high-quality digital images from analog scanning electron microscopes |
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Authors: | Gebert & Preiss |
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Affiliation: | Department of Functional and Applied Anatomy, Laboratory of Cell Biology and Electron Microscopy, Medical School of Hannover, 30623 Hannover, Germany |
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Abstract: | A method is described for converting video signals of analog scanning electron microscopes (SEMs) into digital images of high quality. A plug-in card commercially available for personal computers is used for the on-line analog/digital conversion. A Windows application program written by the authors, together with low-level software drivers supplied with the plug-in card, allow digital images to be recorded, to be displayed simultaneously on the computer monitor and to be saved as a file in a standardized format. Compared to conventional photographic images obtained from the SEM camera system, the digital images possess superior sharpness of outline, excellent image definition, diminished noise and well-defined grey-scale tones. This method provides SEM images of high quality for less than $1000 from most older analog SEMs. In addition, the advantages of digital image processing can be applied to analog SEMs, including contrast enhancement, digital filtering and multichannel recording. |
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Keywords: | Analog/digital converter digital imaging personal computer scanning electron microscopy Windows software application |
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