Concerns for the determination of free fatty acid in cottonseed |
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Authors: | P. J. Wan D. R. Pakarinen P. J. Wakelyn |
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Affiliation: | (1) SRRC/ARS/USDA, P.O. Box 19687, 70179-0687 New Orleans, LA;(2) National Cotton Council, 20036 Washington, DC |
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Abstract: | The official AOCS method for the determination of free fatty acid (FFA) in cottonseed requires dehulling the seed, grinding the meats with a 12-blade food processor, and extracting the ground meats in a butt tube with three portions of room-temperature petroleum ether. The extracted oil, after desolventization, is then titrated with NaOH to the end point of phenolphthalein in a mixed solvent of isopropanol and hexane. Our study showed that this procedure tends to underestimate the amount of FFA present in the oil of cottonseed by as much as 11.5%. It was also found that to obtain consistent and accurate FFA content, a desirable particle size is smaller than 10 mesh (preferably <14 mesh), minimum extraction temperature should be no less than 40°C (preferably greater than 50°C), and the extraction time should be longer than 2 h in a Soxhlet extractor. |
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Keywords: | Butt tube cottonseed free fatty acid Soxhlet |
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