Comparative study on biohydrogen production by newly isolated Clostridium butyricum SP4 and Clostridium beijerinckii SP6 |
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| Affiliation: | 1. Federal State Institution «Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences» Leninsky Prospect, 33, Building 2, 119071, Moscow, Russian Federation;2. Federal Government Budgetary Institution of Science. “Federal Scientific Agroengeneering Centre VIM”, 1st Institutskiy Proezd, Building 5, 109428, Moscow, Russian Federation;3. Russian University of Transport, 127994, St. Obraztsova, 9, Bldg. 9, Moscow, Russia;1. Faculty of Polymer Science, Iran Polymer and Petrochemical Institute, P.O. Box 14965/115, Tehran, Iran;2. Department of Mechanical Engineering and Materials, Universidad de Seville, P.O. Box 159163-4311, Seville, Spain;1. Engineering Research Center of Ministry of Education for Fine Chemicals, Shanxi University, Taiyuan 030006, China;2. School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;1. Federal Scientific Agroengineering Center VIM, 1st Institutsky Proezd, 5, 109428, Moscow, Russia;2. Russian University of Transport, 127994, Moscow, Russia;1. College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, PR China;2. College of Materials Science and Engineering, Qiqihar University, Qiqihar 161006, PR China;3. College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China;1. University of Belgrade, Faculty of Physical Chemistry, Studentski trg 12–16, 11158, Belgrade, Serbia;2. University of Belgrade, Faculty of Chemistry, Studentski trg 12–16, 11158, Belgrade, Serbia;3. University of Belgrade, “VINČA” Institute of Nuclear Sciences – National Institute of the Republic of Serbia, Mike Petrovića Alasa 12–14, 11000, Belgrade, Serbia;4. Center of Physics and Engineering of Advanced Materials, Laboratory for Physics of Materials and Emerging Technologies, Chemical Engineering Department, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal |
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| Abstract: | The hydrogen-producing bacteria SP4 and SP6 were isolated from the compost and identified by 16S rRNA gene sequencing as Clostridium butyricum and Clostridium beijerinckii, respectively. A comparative study on the biohydrogen-producing activity of the isolated strains was carried out using mono-, di- and tri-saccharides belonging to both hexoses (maltose, glucose, mannose, fructose, lactose, galactose, sucrose, raffinose, cellobiose) and pentoses (xylose). To assess the biotechnological significance, real wastewater rich in sugars (cheese whey, confectionery wastewater, sugar beet processing wastewater) was also used as a substrate. C. butyricum SP4 fermented sugars with a yield of 0.93–1.52 mol H2/mol hexose (pentose); the maximum yield was obtained from fructose, the minimum – from raffinose and cellobiose. The most preferred substrate for C. beijerinckii SP6 was sucrose with a yield of 1.76 mol H2/mol hexose, while cellobiose yielded only 0.64 mol H2/mol hexose. Overall, the efficiency of converting wastewater to H2 by C. butyricum SP4 was also slightly lower (66–93 ml H2/g chemical oxygen demand (COD)) than that of C. beijerinckii SP6 (76–103 ml H2/g COD). Even though the main soluble metabolite products (SMPs) for both isolates were acetate and butyrate, C. butyricum SP4 also produced a significant amount of ethanol (up to 21.5% of SMPs) and formate (up to 32.5% of SMPs), and C. beijerinckii SP6 – lactate (up to 25% of SMPs). A distinctive feature of C. beijerinckii SP6 was a significantly lower (almost 2 times) yield of SMPs, while C. butyricum SP4 had a higher rate of H2 production according to the results obtained from the kinetic study using the modified Gompertz equation and the first order equation. Analysis of Spearman's rank correlation coefficients revealed a statistically significant relationship between the kinetic parameters of H2 production and the concentration of butyrate and the final pH of the medium for C. butyricum SP4, and with the concentration of ethanol for C. beijerinckii SP6. These findings provide valuable information on the metabolic capabilities of the most studied hydrogen-producing representatives of the Clostridium genus for their use in optimizing the technology for biohydrogen production by dark fermentation of various organic wastes. |
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| Keywords: | Dark fermentation Wastewater Metabolic pathway Process kinetics |
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