Aqueous extraction of corncob xylan and production of xylooligosaccharides |
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| Affiliation: | 1. School of Food Science and Technology, Southern Yangtze University, Wuxi, Jiangsu 214036, China;2. Department of Food and Animal Sciences, Alabama A&M University, 4900 Meridian Street, Normal, AL 35762, USA;1. Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor Bahru, Johor Darul Takzim, Malaysia;2. Department of Chemical and Process Engineering, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia;3. Advanced Membrane Technology Research Centre, Universiti Teknologi Malaysia, 81310, Skudai, Johor Bahru, Johor Darul Takzim, Malaysia;4. Technology and Heritage Department, Faculty of Science, Technology and Human Development, Universiti Tun Hussein Onn Malaysia, 86400, Parit Raja, Batu Pahat, Johor Darul Takzim, Malaysia;1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Engineering, China Agricultural University, Beijing 100083, China;2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | Xylooligosaccharide, which is usually produced from xylan by enzymatic hydrolysis, is a newly developed functional oligosaccharide having many beneficial biomedical and health effects. In this study, a process for producing xylooligosaccharides from corncob was developed. The procedure consisted of soaking the corncob in 1.0 g/l H2SO4 at 60 °C for 12 h, followed by filtrating and washing with tap water to pH 6 and steaming at 135–140 °C for 0.5 h. The steamed corncob was mashed and hydrolysed by xylanase. The cumulative yield of xylooligosaccharides after enzymatic hydrolysis was close to 67.7 g/100 g by steaming (based on xylan in raw material), and the purity of xylooligosaccharides was over 70 g/100 g (based on the total sugars in the hydrolysate). This study demonstrated that the xylan–lignin complex present in the corncob was affected during steaming at 135 °C for 30 min. Almost all of the xylan was removed from the lignin after steam application and subsequent enzymatic hydrolysis. |
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